產(chǎn)品名稱:EC肉湯
Product name:E.Coli Broth
產(chǎn)品用途:用于糞大腸菌群、大腸桿菌的測定。
Product usage:For the determination of fecal coliform and Escherichia coli.
檢驗(yàn)原理:
Test principle:
胰蛋白胨提供碳氮源;3 號(hào)膽鹽抑制革蘭氏陽性菌,特別抑制革蘭氏陽性桿菌和糞腸球菌;乳糖是可發(fā)酵的糖類;磷酸氫二鉀和磷酸二氫鉀為緩沖劑;氯化鈉可維持均衡的滲透壓。
Tryptone provides carbon and nitrogen source. Bile salt 3 inhibits gram-positive bacteria, especially Gram-positive bacteria and enterococcus faecalis. Lactose is a fermentable sugar; Potassium dihydrogen phosphate and potassium dihydrogen phosphate are buffer agents. Sodium chloride can maintain a balanced osmotic pressure.
配方成分
Formula ingredients
配方 Formula |
含量(g/L) Content (g/L) |
胰蛋白胨 Tryptone |
20.0 |
乳糖 Lactose |
5.0 |
氯化鈉 Sodium chloride |
5.0 |
磷酸氫二鉀 Dipotassium hydrogen phosphate |
4.0 |
磷酸二氫鉀 Potassium dihydrogen phosphate |
1.5 |
三號(hào)膽鹽 Bile iii salt |
1.5 |
pH |
6.9±0.1 25℃ |
使用方法
Usage
稱取本品37.0g,加熱攪拌溶解于 1000ml蒸餾水中,分裝到有倒立發(fā)酵管的16mm*150mm試管中,每管8ml,121℃高壓滅菌15分鐘,備用。
Weigh 37.0g of this product, stir and dissolve it in 1000ml distilled water, and divide it into 16mm*150mm test tubes with inverted fermentation tubes, 8ml each tube, autoclave at 121℃ for 15 minutes, and set aside.
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